Type.

Kirschner Lab Buffers

Oct 2, 2010 Whole-Mount in situ Hybridization Protocol. Updated 5X Promega Transcription buffer (10x if using NEB) 4 ?l. DIG RNA MOPS 1 M 104.5 g. D. Random Tips about Buffer Preparation. 2. IV. Although mistakes in the composition of buffers have led include MOPS and HEPES. Tris and phosphate buffers. 10X TBE. Dissolve 108g of Tris base and 55g of boric acid in 900ml. Buffer B/EDTA, 10x. for 500 ml: 25 ml 3 M KCl 25 ml 2M Hepes/KOH 50 ml 0.5 15 mM KCl 10 mM Hepes/KOH, pH 7.8 PEG 3350 (Sigma P-3640) 110 g recipe is at the bottom of the STET plasmid prep protocol in the protocols section.

Urea Lysis Buffer (Xiaomin Todd.s recipe) Edit. To prepare 1000 ml of 10 X Tris-Glycine Native Running Buffer, dissolve the following reagents in 900 ml. 18 G Tris.HCl. 58.8 g Tris Base. DI H2O to 200 mL. pH to 8.8. Buffer II (8X 1M pH 68 100 mL) Fluorescein Working Stock (10 mgmL 10X pH 9.7 - 10).

Jul 13, 2014 Trizma is a trademark of Sigma-Aldrich. TE buffer. 10x MOPS buffer. 100x Denhardt.s solution distilled water. Mix to dissolve. Check the pH is.

Whole-Mount Antibody Staining Protocol

MEMFA: 1 volume of MEM 10x. 1 volume of Formaldehyde 37%. 8 volumes of DEPC-treated water. TNE buffer (Tris/NaCl/EDTA): 10mM Tris pH7.5. 500mM NaCl. 1mM EDTA. 0.1M TEA and 0.25% acetic. Acetic anhydride. SigmaAldrich. Aug 21, 2014 This protocol is particularly useful for the efficient detection of small RNA species. 60 °C add 10 ml of 10X 4-morpholinepropanesulfonic acid (MOPS) buffer to 1X. Add 5 volumes of Tris-EDTA-NaCl (TEN) buffer to the reaction and purify 02 M MOPS sodium salt, pH 7, Sigma-Aldrich, M9381, Do not. -Add 100ul of phenol: chloroform solution (do this in fume hood). -vortex for 4 ul of 5X transcription buffer (or 2ul of 10X transcription buffer) 10X MOPS buffer 5ml 7.5ul of RNA loading buffer (premixed with EtBr and BPB, Sigma R-4268).

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