Dec 13, 2012 buffer (80 mM PIPES/KOH, pH 6.8, 1 mM EGTA, 1 mM MgCl2. if not specifically buffer A (1 mM MgCl2, 10 mM MOPS pH 7.4, 0.1 mM EGTA, 1. Abcam) using HyNic protein conjugation toolkit (SoluLink) according to the. Oct 26, 2009 Blood from a razor nick was diluted in buffer (saponin 0.3%, proteins in experimental buffer (125 mM KCl, 10 mM Tris-MOPS, pH 7.4, Protocol details, including peptide sequence, have been previously 15 ?L of 200 ?M peptides in T-EB (300 mM Trehalose, 10 mM Hepes-KOH pH 7.7, 80 mM KCl. Oct 15, 2013 for 20 h and incubated for 45 min at 281C in zymolyase buffer (1.2 M sorbitol, 20mM 1mM EDTA, 10mM MOPS-KOH, pH 7.2) at A 801C. For the or a rabbit polyclonal antibody against ?-tubulin (Abcam) and the respective soaked with solution containing 10% sucrose and 20 mM MnCl2 as previously.
Sep 7, 2013 Cell extracts preparation, Western blo
